Hygromycin B, Streptomyces sp. – CAS 31282-04-9 – Calbiochem

Descrição detalhada : Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units., Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent., Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).The analytical data listed below will vary from lot to lot.Bioassay: 350-450 U/mgConcentration: 444,600 U/ml; 394 mg Hygromycin B/mlDensity: 1140-1155 mg/ml% solids: 34.6%Fill volumes:100,000 U = 225 µl1,000,000 U = 2.25 ml 10,000,000 U = 22.5 ml250,000 U = 562 µl5,000,000 U = 11.25 ml, Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
Sinónimos : Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem
Fórmula molecula r: C20H37N3O13
Armazenamento : 2-8C; 2-8C
Embalagem : 1X5MU