ChemiScreen Membrane Preparation Recombinant Human M5 Muscarinic Acetylcholine Receptor

Descrição detalhada : The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M5 receptor, along with the M1 and M3 receptors, signal through Gq/11 and subsequent release of Ca++ from the ER (Caulfield and Birdsall, 1998). Although M5 is expressed in the CNS at relatively low levels, it appears to be the only muscarinic acetylcholine receptor expressed in midbrain dopaminergic neurons, and it has been shown to mediate dopamine release from these neurons. The M5 receptor is also expressed in blood vessels in the brain and the periphery, and studies with M5 knockout mice demonstrate that M5 mediates the vasodilatory action of acetylcholine on cerebral microvessels. Further studies with M5 knockout mice also indicate a role for M5 in the rewarding effects of cocaine and morphine (Wess, 2004). Chemicon's M5 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of M5 interactions with its ligands. The membrane preparations exhibit a Kd of 0.16 nM for [3H]-Scopolamine methyl chloride (NMS). With 10 mug/well M5 Membrane Prep and 1 nM [3H]-NMS, greater than 5-fold signal-to-background ratio was obtained., Full-length human CHRM5 cDNA, encoding M5
Armazenamento : -80°C
Embalagem : 1X1EA