dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA

Descrição detalhada : This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide (EF1a-dCas9-VP64-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma’s lentiviral dCas9-VP64 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing dCas9-VP64 for CRISPR based transcipritonal activation and genome wide gain of function screening. Sigma’s lenti-dCas9-VP64 plasmid is one part of a three part CRISPR system with individual dCas9-VP64, MS2-p65-HSF1 and gRNA expression vectors. To order gRNA in any format click here
Armazenamento : -20C
Embalagem : 1X10UG