LentiBrite RFP-LC3 Lentiviral Biosensor

Descrição detalhada : Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infections. Members of the LC3 family play a key role in the maturation of the autophagosome, the central organelle of autophagy. LC3 precursors, diffusely distributed in the cytosol, are proteolytically processed to form LC3-I. Upon initiation of autophagy, the C-terminal glycine is converted to LC3-II by addition of a phosphatidylethanolamine by Atg5-Atg12. LC3-II translocates rapidly to nascent autophagosomes in a punctate distribution. DNA constructs encoding fluorescent proteins fused to LC3 are widely employed for introduction into cells for monitoring autophagosome formation by fluorescence microscopy. EMD Millipore's LentiBrite RFP-LC3 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types., Also available: LentiBrite GFP-p62 Lentiviral Biosensor! Click HereAlso available: LentiBrite RFP-p62 Lentiviral Biosensor! Click HereRead our application note in Nature Methods!http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8620.pdf(Click Here!)Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click HereBiosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity. EMD Millipore is introducing LentiBrite Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.• Pre-packaged, fluorescently-tagged with GFP & RFP • Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods • Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells • Non-disruptive towards cellular function EMD Millipore’s LentiBrite RFP-LC3 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types.
Sinónimos : Microtubule-associated proteins 1A/1B light chain 3, Autophagy-related protein LC3, Autophagy-related ubiquitin-like modifier LC3, MAP1A/MAP1B light chain 3, Microtubule-associated protein 1 light chain 3
Armazenamento : -80°C
Embalagem : 1X1EA