LentiBrite PSD95-GFP Lentiviral Biosensor

Descrição detalhada : Read our application note in Nature Methods!http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8620.pdf(Click Here!)Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click HereBiosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity. EMD Millipore is introducing LentiBrite Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.• Pre-packaged, fluorescently-tagged with GFP & RFP • Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods • Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells • Non-disruptive towards cellular function EMD Millipore’s LentiBrite PSD-95-GFP lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of PSD dynamics in neuronal cells., The postsynaptic side of an excitatory synapse contains an electron- and protein-dense region, the postsynaptic density (PSD), that positions neurotransmitters adjacent to sites of presynaptic neurotransmitter release, and also concentrates signaling molecules. MAGUKs (membrane-associated guanylate kinases) serve both as central organizers of the PSD and as signaling molecules. One such MAGUK, PSD-95, is associated with the dendritic spines of mature synapses, and is relatively immobile compared to other MAGUKs. PSD-95 fused to fluorescent proteins has been employed to quantify turnover and mobility of PSD-95. EMD Millipore's LentiBrite PSD-95-GFP lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of PSD dynamics in neuronal cells.
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Embalagem : 1X1EA