MEC1 Mouse Embryonic Epicardial Cell Line

Descrição detalhada : Heart development is guided by mitogenic factors secreted by the epicardium, a cell layer that overlays the myocardium in the mid-gestation embryonic heart. Despite the importance of these factors in directing cardiomyocyte proliferation and morphogenesis, they are not well-characterized. Cellular models of the embryonic epicardium are thus essential for gaining a comprehensive understanding of the mechanisms of cardiac development., MEC1 is an immortalized mouse embryonic epicardial cell line that does not express myocardial or endothelial cell lineages. MEC1 cells retain the morphology of early primary epicardial cells and express epicardium-specific markers including epicardin (Tcf21), Tbx18 and cytokeratin-18. MEC1 cells localized ZO-1 and beta-catenin in adherens junctions at the cell membrane and possessed organized actin cytoskeletal filaments. The native physiological features of the MEC1 cell line contribute to its suitability for addressing a wide range of questions in cardiac developmental biology.Source:Non-GMO. MEC1 is a stable cell line derived from primary cultures of E13.5 wild-type embryonic ventricles of an ICR/CD1 strain mouse. Individual colonies that retained early epicardial cell morphology underwent several iterative rounds of subcloning.Reference:Li P, Cavallero S, Gu Y, Chen TH, Hughes J, Hassan AB, Bruening JC, Pashmforoush M, Sucov HM (2011). IGF signaling directs ventricular cardiomyocyte proliferation during embryonic heart development. Development 138(9): 1795-17805.
Armazenamento : -140C Degree Freezer
Embalagem : 1X1EA