INS-1 832/3 Rat Insulinoma Cell Line

Descrição detalhada : Glucose-stimulated insulin secretion (GSIS) is potentiated by pancreatic beta cells and is critical to the physiological control of blood glucose levels. Insulin secretion is impaired in type 2 diabetes, and insight into the mechanisms and regulation of insulin secretion is fundamental to understanding the roles of beta cells in metabolic disease., The rat insulinoma cell line INS-1 is a well-established model for studies of pancreatic islet beta-cell function ; however, the GSIS response of INS-1 cells may decrease over time. The INS-1 832/3 cell line is a subclone of INS-1 that was selected for robust GSIS, producing and secreting both rat and human insulin. INS-1 832/3 harbors a human insulin expression cassette allowing for human insulin secretion to be maintained over extended passages with selection . INS-1 832/3 cells are widely used to study the mechanisms of cellular insulin secretion, storage and synthesis. INS-1 832/3 cells may be characterized by granular staining for synaptotagmin, as described for the parental cell line . The INS-1 832/3 cell line exhibits the unique feature of additional insulin secretion in response to natural incretin hormones such as glucagon-like peptide 1 (GLP1), pituitary adenylate cyclase-activating peptide (PACAP), and gastric inhibitory peptide (PIP) , making INS-1 832/3 a valuable tool for physiologically relevant investigations of insulin regulation. Source: INS-1 832/3 is a derivative of INS-1 cells originally established from an x-ray induced insulinoma in rat1. The INS-1 832/3 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human insulin expression plasmid carrying a geneticin (G418)-resistance marker for selection .
Sinónimos : INS1 832/3, INS-1 (832/3), 832/3
Armazenamento : -140C Degree Freezer
Embalagem : 1X1EA