Dpn I

Descrição detalhada : IsoschizomersThe enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.Methylation sensitivityThe enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.Typical ligation and recutting assayDpn I fragments obtained by complete digestion of 1mug pBR322 DNA are ligated for 16 hours at +4C with 1U T4 DNA Ligase in 10mul buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 x Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis., Dpn I requires methylation of adenine residues for activity and thus digests only GmATC sequences containing N6-methyladenine. Such methylated sequences are produced by dam methylase. This requirement for methylation distinguishes Dpn I from Mbo I, which is inhibited by dam methylation, and Sau3A I, which is not influenced by dam methylation. Dpn I also cleaves at a different position on the recognition sequence than Mbo I and Sau3A I.
Sinónimos : Dpn I
Armazenamento : -20C
Embalagem : 1X1EA