Taq DNA Polymerase, 1 U/mul

Descrição detalhada : The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 C) and temperatures around +75 C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates, and can be used to label DNA-fragments either with radionucleotides, digoxigenin, fluorescein or biotin.The high processivity, absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing, especially where the resolution of secondary structures plays a major role.
Sinónimos : dna amplification; pcr; polymerase; primer extension
Armazenamento : -20C
Embalagem : 1X1EA