Angiotensin Converting Enzyme-2, ACE2

Descrição detalhada : Angiotensin Converting Enzyme-2 (ACE2) is a membrane-associated and secreted enzyme that is principally expressed on endothelium. In humans, ACE2 occurs mainly in endothelium of heart, kidney, and testis, with other expression in coronary vessel smooth muscle and kidney tubular epithelium ACE2 is a component of the renin-angiotensin system (RAS) that provides protective effects in peripheral tissues. Coronaviruses such as SARS-CoV-2 and SARS-CoV-1 use the host ACE2 as a co-receptor to gain intracellular entry into the lungs and brain. The virion expresses a protein termed spike, which directly binds to the extracellular domain of ACE2. A specific region in the spike protein serves as the receptor binding domain (RBD). The ACE2:spike interaction has a high affinity of 15 nM.Recombinant human ACE2 is expressed in human HEK 293 cells as a glycoprotein with a C-terminal FLAG(R) tag and his-tag with a calculated molecular mass of 85.9 kDa. The DTT-reduced protein migrates as a 90-120 kDa polypeptide on SDS-PAGE due to glycosylation.This ACE2 is labelled with biotin, to form Angiotensin Converting Enzyme-2. This ACE2 biotin-tagged may serve as a useful tool for binding experiments while detecting protein: protein interaction assays using binding partners such as CoV spike protein. The degree of biotinylation is >=90%. This ACE2-biotin can be visualized with streptavidin-conjugated probes (e.g., Streptavidin, HRP conjugate catalog No. 18-152).
Sinónimos : ACE-related carboxypeptidase; ACE2; Angiotensin-converting enzyme homolog (ACEH); COVID-19 receptor; Metalloprotease MPROT15; SARS-COV2 receptor
Armazenamento : -20C
Embalagem : 1X25UG