ONPG Disks

Descrição detalhada : ONPG Disks are used to detect the presence of beta-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: beta-galactoside permease, which catalyzes transport of lactose into the cell, and beta-galactosidase, which breaks down lactose into galactose and glucose. beta-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-beta-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.
Sinónimos : beta-Galactosidase Test Disks; 2-Nitrophenyl-beta-D-galactopyranoside Disks
Armazenamento : 2-8C
Embalagem : 1X50DISCS