HyasKit

Descrição detalhada : The Hyas MicroMap Kit provide a method for small molecule intracellular target identification via photocatalytic proximity labeling. The kit contains a catalyst that can be coupled to an acid on almost any small-molecule ligand. The subsequent ligand-Iridium conjugate can be used to perform multiple target identification experiments.The following kit provides enough Ir-catalyst to synthesize 20 muMoles of iridium-ligand conjugate and enough Diazirine-PEG3-Biotin to perform 10 proteomics experiments. The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand-iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis. To link to an amine on a small molecule (instead of an acid), the Dione Kit is available.
Sinónimos : Microenvironment mapping technology; Photoinduced proximity labeling kit; Protein-protein interaction elucidation technology; Small molecule-photocatalyst conjugate labeling
Armazenamento : -20C