Abberior(R) STAR 635P, NHS ester

Descrição detalhada : Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.Abberior STAR 635P can substitute Atto(TM) 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.Best results are obtained with freshly prepared samples.Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.Key Features. First phosphorylated dye . Unmatched, background free STED imaging contrast. Verified in the Leica STED systemAbsorption Maximum, lambdamax: 638 nm (PBS, pH 7.4)Extinction Coefficient, epsilon(lambdamax): 120'000 M-1cm-1 (PBS, pH 7.4) Correction Factor, CF260 = epsilon260/epsilonmax: 0.21 (PBS, pH 7.4)Correction Factor, CF280 = epsilon280/epsilonmax: 0.40 (PBS, pH 7.4)Fluorescence Maximum, lambdafl: 651 nm (PBS, pH 7.4)Recommended STED Wavelength, lambdaSTED: 750 - 780 nm Fluorescence Quantum Yield, eta: 0.90 (PBS, pH 7.4)Fluorescence Lifetime, tau: 3.3 ns (PBS, pH 7.4)
Armazenamento : -20C
Embalagem : 1X1MG