AccuChIP Trimethyl-Histone H3 Internal Control(Lys27) Chromatin Immunoprecipitation

Descrição detalhada : AccuChIP Chromatin Immunoprecipitation Internal Controls are designed to improve the accuracy of ChIP experiments. This simple to spike-in reagent is composed of a target-specific peptide sequence covalently bound to a uniquely designed double stranded nucleic acid sequence. Because AccuChIP controls mimic the behavior of cross-linked chromatin, by simply adding a known amount of the AccuChIP reagent to your chromatin and performing a standard ChIP reaction followed qPCR detection you can improve the accuracy and reproducibility of your ChIP experiments. Features and Advantages. Simple to use - just add to chromatin either before or after sonication . Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals . Target specific control - uses the same antibody used for your ChIP experiment . Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling . Easily incorporated into virtually any ChIP protocol . Readily detected by qPCR . Easily incorporated into ChIP-seq libraries . Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity
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Embalagem : 1X1EA