Anti-Aggrecan Antibody, MMP Cleaved, clone AF-28, Ascites Free

Descrição detalhada : Aggrecan core protein (UniProt P16112; also known as Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP) is encoded by the ACAN (also known as AGC1, CSPG1, MSK16, SEDK) gene (Gene ID 176) in human. Aggrecan is the major extracellular matrix (ECM) proteoglycan in articular cartilage that, together with type II collagen, provides the cartilage with its mechanical properties of reversible compressibility. Aggrecan consists of two globular domains (G1 and G2) and an IGD (interglobular domain) sequence in between, followed by an extended region before the third globular domain (G3). The region between G2 and G3 is heavily substituted with negatively charged sGAG (sulfated glycosaminoglycan) and is further subdivided into the KS (keratan sulfate), CS1 (chondroitin sulfate region 1) and CS2 regions. Degradation of cartilage ECM, including aggrecan, is a hallmark in arthritic diseases and in joint injuries. Multiple aggrecan cleavage sites have been characterized, including those in G1 domain (by cathepsin K), IGD (by aggrecanases, calpain, cathepsins B and K), KS (by calpain and MMPs), CS1 (by calpain and MMPs), and CS2 (by aggrecanases, calpain, cathepsin D and MMPs). In addition, different MMPs exhibit differential affinities toward aggrecan, the IGD IPEN-FFGV site is cut by MMPs 1-3, 7-9, 12-16, 19 and 20, the CS1 region in bovine aggrecan is cut at multiple GVED-I/(L)SGL sites by MMP-3, the end of the CS2 region in bovine aggrecan is cut at RPAE-ARLE by MMPs 2, 3, 7 and 12. Aggrecan proteolysis in the IGD results in decreased tissue water-holding capacity due to a loss of sGAG-containing chains. On the other hand, aggrecan proteolysis in the CS1 and CS2 regions is a natural turnover process in mature cartilage and does not affect cartilage function.
Sinónimos : Aggrecan core protein, MMP-cleaved, Cartilage-specific proteoglycan core protein, MMP-cleaved, Chondroitin sulfate proteoglycan 1, MMP-cleaved, Chondroitin sulfate proteoglycan core protein 1, MMP-cleaved, CSPCP, MMP-cleaved
Armazenamento : +2°C to +8°C
Embalagem : 1X1EA