Anti-CEP Antibody, clone Das-1 (7E12H12)

Descrição detalhada : The monoclonal antibody (mAb) Das-1 was originally desiganted as clone 7E12H12 and was thought to target a 40 kDa colon-specific tropomyosin (TM) isoform or a TM-related protein. It was later found that this clone specifically immunoprecipitated a high molecular weight chaperone protein that targets tropomyosin 5 (TM5) in human colon epithelial cells and subsequently named Colon Epithelial Protein (CEP). The Das-1 target is a glycoprotein with variable apparent sizes depending on the level of glycosylation. The target glycoprotein immunoprecipitated from the plasma membrane of DLD-1 colon cancer cells appears with an apparent molecular size of 185 kDa, while the target isolated from LS-180 colon cancer cells migrates with a size range extends to greater than 200 kDa. Das-1/CEP immunoreactivity is reported to be associated with Barrett's metaplasia, Barrett's adenocarcinoma, as well as with gastric adenocarcinomas and carcinoma-associated intestinal metaplasia, but no reactivity has been found in normal esophageal, gastric or small intestinal mucosa. When tested by ELISA and imunoblotting against recombinant human tropomyosins isoforms 1-5, none of five hTM isoforms reacted with mAB DAS-1. As determined by immunofluorescence and FACS analyses, CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
Sinónimos : Colon epithelial protein, CEP, Das-1 protein
Armazenamento : +2°C to +8°C
Embalagem : 1X1EA