Anti-Medullary epithelium Antibody, clone ER-TR5

Descrição detalhada : The thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTEC) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. In murine species, mTEC are further divided into mTEC low and mTEC high according to the expression levels of several maturation molecules, such as MHCII and CD80. mTEC low cells can serve as precursors for mature mTEC high cells. mTECs have also been classified into four major subsets, termed mTEC I-IV depending on their distinct transcriptional and molecular characteristics. mTEC I and II subpopulation appear early and can be detected at E18.5 and are most proliferative cells. mTEC III are detected in thymus at 4-weeks. mTEC IV are visible on day 6 in neonatal mice. The function of mTECs is dependent on their characteristic features such as ectopic expression of peripheral tissue restricted antigens and their master regulator-autoimmune regulator (Aire), expression of various chemokines and cytokines. Lymphotoxin beta receptor (LTbetaR) is reported to be an essential regulator of multiple mTEC subsets within the mTEC low compartment. It controls thymic tolerance by regulating the frequency and makeup of intrathymic dendritic cells that are required for effective thymocyte negative selection. Clone ER-TR5 is shown to exclusively react with mTECs. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Lucas, B., et al. (2020). Nat. Commun. 11; Article 2198; Cosway, EJ., et al. (2017). J. Exp. Med. 214(11); 3183-3195; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529).
Sinónimos : mTEC
Armazenamento : +2°C to +8°C