Anti-Rabbit IgG, Native-Peroxidase antibody, Mouse monoclonal
REF : R3155-200UL
Marca : Sigma-Aldrich
Descrição :Anti-Rabbit IgG, Native-Peroxidase antibody, Mouse monoclonal clone RabT-50, purified from hybridoma cell culture
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Categoria : Antibodies
Descrição detalhada : Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies., Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Sinónimos : Mouse Anti-Rabbit IgG
Armazenamento : -20C
Embalagem : 1X200UL
Sinónimos : Mouse Anti-Rabbit IgG
Armazenamento : -20C
Embalagem : 1X200UL
