beta-Galactosidase Reporter Gene Staining Kit

Descrição detalhada : ?-galactosidase (?-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of ?-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of ?-gal like O-nitrophenyl-?-D-galactopyranoside (ONPG). ?-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the ?-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Sinónimos : beta-galactosidase; LacZ
Armazenamento : -20C
Embalagem : 1X1KIT