beta-Galactosidase stain

Descrição detalhada : The gene of beta-galactosidase from E. coli is widely used as a reporter gene assay marker. Although X-gal is well known reagent to detect beta-galactosidase in cell or tissue samples, the assay using these reagents require to fix cells or tissues due to the poor cell-permeability. In addition, so far developed the assay using fluorescence reagents can not clearly differentiate beta-galactosidase-expressed cells or regions.To overcome these issues, Urano, Kamiya and co-workers have successfully developed a stain ideally possesses cell-permeability and the ability to retain in intracellular region.1)By the enzymatic reaction, the beta-Galactosidase stain immediately forms a quinone methide that acts as electrophile when proteins containing nucleophilic functional groups nearby the molecules. By the probe undergoes the reaction with a protein, the conjugates become fluorescent compounds. Thus, it allows a single-cell analysis because it does self-immobilizing to the intracellular proteins.Exciation Maximum: 530 nm (+/- 10 nm)Emission Maximum: 550 nm (+/- 10 nm)Reference: 1) Y. Urano, M. Kamiya, T. Doura, WO 2015174460, A1, (19, November, 2015). Find more infomation here 94433
Sinónimos : 6'-(Diethylamino)-4'-(fluoromethyl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl beta-D-galactopyranoside; SPiDER-betaGal; SPiDER-beta Gal; (2S,3R,4S,5R,6R)-2-{[3'-(Diethylamino)-5'-(fluoromethyl)-3H-spiro(isobenzofuran-1,9'-xanthen)-6'-yl]oxy}-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
Fórmula molecula r: C31H34FNO8
Armazenamento : 2-8C
Embalagem : 3X20UG