Caspase-3 Cellular Activity Assay Kit

Descrição detalhada : Caspase-3 (also known as CPP32, apopain, and Yama), a member of the interleukin-1beta converting enzyme (ICE) family of cysteine proteases, is composed of 17 and 12 kDa subunits derived from a common proenzyme, pro-caspase-3. It is activated during apoptotic signaling events by upstream proteases including caspase-6, caspase-8 (FLICE), and cytotoxic T-cell-derived granzyme B. Caspase-3 is one of the principal caspases found in apoptotic cells. Targets of caspase-3 cleavage include poly(ADP-ribose) polymerase (PARP), nuclear lamins, gelsolin, and others. Caspase-3 is a potential therapeutic target., A convenient assay kit useful for measuring the caspase-3 and caspase-3-like activities in cell extracts using either a colorimetric or fluorometric substrate. Provided in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. In addition, caspase-3 is included for use as a positive control, for testing cell extracts for endogenous inhibitors, or for comparing effects of exogenous inhibitors on cellular activity versus the activity of the purified enzyme.
Armazenamento : -70C
Embalagem : 1X1KIT