CHAPS, Molecular Biology Grade – CAS 75621-03-3 – Calbiochem

Descrição detalhada : Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON(TM) X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14., Conductivity (1.0 M, 24C): <100 µmhos., Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON(TM) X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.
Sinónimos : CHAPS; 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate; 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
Fórmula molecula r: C32H58N2O7S
Armazenamento : 15-25C
Embalagem : 1X250GM