ChemiScreen Membrane Preparation Recombinant Human TBXA2R (TP) Thromboxane Receptor

Descrição detalhada : TP receptors (Thromboxane A2 receptors) are widely distributed among different organ systems and have been localized on both cell membranes and intracellular structures. TP receptors belong to G protein-coupled receptor family (Hirata et al., 1991). Activation of TP receptors induces platelet aggregation, vascular and respiratory smooth muscle constriction, and enhances mitogenic responses of vascular smooth muscle cells that are stimulated by growth factors (Ali et al, 1993; Hanasaki et al., 1990). The human TP receptor is encoded by a single gene that is alternatively spliced at the carboxyl terminus, resulting in two isoforms, TPalpha (343 residues) and TPbeta (407 residues). Both isoforms couple to Gq pathway, but couple oppositely to adenylate cyclase. The cDNA encoding human TPalpha has been stablely expressed in the Chem-1 host, which supports high levels of recombinant receptor expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. The membrane preparations exhibit a Kd of 9.19 nM for [3H]-SQ 29548. With 10 nM [3H]-SQ 29548, 10 ug/well TP Membrane Prep yields greater than 5-fold signal-to-background ratio., Human Full-length human TBXA2R cDNA encoding the TP short form (TPalpha)
Armazenamento : -80°C
Embalagem : 1X1EA