CRISPR GUS GAPDH Reporter Control for Dicots

Descrição detalhada : All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS ReporterCRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter. Arabidopsis seedlings were germinated in 6 well tissue culture plates The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product
Armazenamento : -20C
Embalagem : 1X1EA