DNA Polymerase I from Escherichia coli lysogenic for NM 964

Descrição detalhada : DNA polymerase I (holoenzyme) has 5'->3' and 3'->5' exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5'->3' exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3'-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5'-end of the template strand) is replaced.
Sinónimos : Kornberg Polymerase
Armazenamento : -20C
Embalagem : 1X500UN