First Strand cDNA Synthesis Kit for RT-PCR (AMV)

Descrição detalhada : The amplification of RNA requires the conversion of the RNA substrate into DNA. This is achieved through the use of a reverse transcriptase such as AMV RT (avian myeloblastis virus reverse transcriptase) or M-MuLV RT (moloney murine leukemia virus reverse transcriptase). The resulting cDNA can be used as a template for a standard PCR.AMV RT synthesizes the new cDNA strand at site(s) determined by the type of the primer used:. at the 3'-end of the poly(A) mRNA when Oligo-p(dT)15is used as a primer,. at nonspecific points along the mRNA template when using the random primer p(dN)6, or. at a site determined by a sequence-specific primer. , The use of AMV RT for first-strand cDNA synthesis has certain advantages. The higher thermostability of this enzyme compared to M-MuLV RT allows the reaction to be performed at +42C, giving higher specificity and better resolution of secondary structures compared to a reaction performed at +37C., The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR.The kit includes Reverse Transcriptase AMV for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA. PCR products that are generated by RT-PCR can be cloned using standard procedures.
Sinónimos : RT-PCR; cDNA sysnthesis
Armazenamento : -20C
Embalagem : 1X1EA