GC-RICH PCR System

Descrição detalhada : The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3'-5' exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH resolution solution allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 mul PCR.Contents. GC-RICH Enzyme Mix , in storage buffer. GC-RICH Reaction Buffer, 5x concentrated, with 7.5 mM MgCl2 and DMSO. GC-RICH Resolution Solution, 5 M. MgCl2 Stock Solution, 25 mM. Water, PCR Grade
Sinónimos : GC-rich | PCR
Armazenamento : -20C
Embalagem : 1X1EA