Gecko2 Human Whole Genome CRISPR Pool, gRNA Only Lenti Particles (Gecko2 vector)

Descrição detalhada : Developed at the Broad Institute by Feng Zhang's lab, the human GeCKO v2 (two vector system) libraries consist of over 100,000 unique gRNAs for gene knock-out in the human genome. Using a dual lenti CRISPR vector wherein the pooled libraries will only express the gRNA (with the lentiGuide-Puro vector), this system produces over 100X higher titer virus compared to version 1. The lentiGuide-Puro pool should be used only in cell lines with Cas9 already integrated (which can be generated using a separate lenti-Cas9-Blast vector). Sigma's lentiviral human GeCKO pool guide RNA only is provided in 8 x 25 ul aliquots at a minimum titer of 5X10^8 TU/ml (measured by a p24 assay). Each species-specific library is delivered as two half-libraries (A and B). It is recommended to screen both A and B libraries together, which will include 6 sgRNAs per gene (3 sgRNAs in each library). Both libraries contain 1000 non-targeting control sgRNAs. The A library also targets miRNAs (4 sgRNAs per miRNA).
Armazenamento : -70C
Embalagem : 1X1EA