GenElute(TM) HP Plasmid Miniprep Kit
REF : NA0150-1KT
Marca : Sigma-Aldrich
Descrição :GenElute(TM) HP Plasmid Miniprep Kit sufficient for 70 preparations
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Categoria : NAA / NAP
Descrição detalhada : An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.The GenElute(TM) HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 mug of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium., We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Sinónimos : GenElute(TM) HP Plasmid Kit; HP miniprep kit; fast miniprep kit; fast plasmid isolation kit; plasmid extraction kit; plasmid isolation kit; plasmid purification kit; ultrafast plasmid isolation kit; Gen Elute
Armazenamento : 15-25C
Embalagem : 1X1KIT
Sinónimos : GenElute(TM) HP Plasmid Kit; HP miniprep kit; fast miniprep kit; fast plasmid isolation kit; plasmid extraction kit; plasmid isolation kit; plasmid purification kit; ultrafast plasmid isolation kit; Gen Elute
Armazenamento : 15-25C
Embalagem : 1X1KIT
