GenomePlex(R) Whole Genome Amplification (WGA) Kit
REF : WGA1-50RXN
Marca : Sigma-Aldrich
Descrição :GenomePlex(R) Whole Genome Amplification (WGA) Kit Kit for whole genome amplification from a variety of DNA sources including FFPE tissue
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Categoria : WGA / WTA
Descrição detalhada : GenomePlex(R) Whole Genome Amplification WGA kit is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 muL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 mug per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI's TaqMan(R) assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.
Armazenamento : -20C
Embalagem : 1X50RXN
Armazenamento : -20C
Embalagem : 1X50RXN
