Glucose Oxidase from Aspergillus niger

Descrição detalhada : Molecular Weight: 160 kDa (gel filtration)pI: 4.2Extinction coefficient: E1% = 16.7 (280 nm)Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for beta-D-glucose with a KM of 33-110 mM.Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes beta-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for beta-D-glucose, solutions of D-glucose can be quantified as alpha-D-glucose will mutorotate to beta-D-glucose as the beta-D-glucose is consumed by the enzymatic reaction.
Sinónimos : beta-D-Glucose:oxygen 1-oxidoreductase; G.Od.; GOx
Armazenamento : -20C
Embalagem : 1X2.5MU