Glutathione Assay Kit

Descrição detalhada : Note: 1 T = 1 test., Oxidative stress occurs in most, if not all, human diseases. Our understanding of the role played by reactive oxygen species in the progression of pathological changes is still evolving. Convenient, accurate and reproducible methodologies to measure oxidative stress parameters have been lacking. Glutathione (γ-glutamylcysteinylglycine or GSH) is a naturally occuring tripeptide whose nucleophilic and reducing properties play a central role in metabolic pathways, as well as in the antioxidant system of most aerobic cells. GSH plays a critical role as a coenzyme with a variety of enzymes including, glutathione peroxidase, glutathione S-transferase, and thiol transferase. GSH also plays major roles in drug metabolism, calcium metabolism, the γ-glutamyl cycle, blood platelet, and membrane functions. In addition, GSH is crucial to a variety of life processes, including the detoxification of xenobiotics, maintenance of the -SH level of proteins, thiol-disulfide exchange, removal of hydroperoxides and free radicals, and amino acid transport across membranes. Physiological values of intracellular GSH generally range from 1 to 10 mM. Although many methods have been described for the assay of GSH, the reliable ones are labor intensive and not easy to use., A spectrophotometric assay kit that is specific for reduced glutathione (GSH) or total mercaptans. Absorbance is read at ~400 nm for GSH and ~356 nm for total mercaptens.
Armazenamento : 2-8C; 2-8C; 2-8C
Embalagem : 1X100T