GSTrap(TM) Fast Flow

Descrição detalhada : GSTrap(TM) FF columns are prepacked Glutathione Sepharose(R) Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap(TM) FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose(R) 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC(TM) System.* See the section for licensing information in our terms and conditions.
Armazenamento : Room Temperature
Embalagem : 1X1EA