High Prime

Descrição detalhada : Enzyme and nucleotide mixture for rapid random-primed labeling of DNA with [32P], [35S], or [3H] dCTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3'-OH termini of the random oligonucleotides as primers., High Prime, a novel labeling mixture, contains optimal concentrations of nucleotides and primers in a highly efficient reaction mix with Klenow enzyme. This convenient "all-in-one" principle of High Prime reduces pipetting steps to a minimum, and increases accuracy and reproducibility of labeling reactions., High Prime is used for rapid random-primed labeling of DNA with [32P], [35S], or [3H]dCTP to a guaranteed specific activity of >2 x 109 dpm/mug. Incubation time is only 10 minutes at +37C.High Prime guarantees efficient labeling of:. DNA amounts ranging from 10 ng to 3 mug in a standard reaction. DNA of different lengths ranging from small restriction fragments to l or cosmid DNA. DNA, supercoiled or linearized. DNA in low melting-point agarose.High Prime-labeled DNA probes are suitable for single-copy gene detection in mammalian DNA in Southern, northern, and dot blots, as well as in colony- or plaque hybridization., Contents:5x concentrated random primer mix: 1 U/mul Klenow polymerase, labeling grade, 0.125 mM dATP, 0.125 mM dGTP, 0.125 mM dTTP in 50% (v/v) glycerol.
Sinónimos : high prime; labeling mix
Armazenamento : -20C
Embalagem : 1X1EA