JumpStart(TM) REDTaq(R) ReadyMix(TM) Reaction Mix

Descrição detalhada : JumpStart(TM) REDTaq(R) ReadyMix(TM)PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma's ReadyMix and REDTaq. The Master mix includes Sigma's JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2x optimized reaction concentrate. Add 25 muL of the ReadyMix to primers, template, and water to a final reaction volume of 50 muL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.
Sinónimos : Hot start PCR master mix; Hot start master mix; Hot start taq master mix
Armazenamento : -20C
Embalagem : 1X100RXN