KOD One(TM) PCR Master Mix

REF : KMM-101NV-5X1ML
Marca : Sigma-Aldrich
Descrição :KOD One(TM) PCR Master Mix Ready-to-use 2X PCR master mix with a hot-start modified KOD DNA polymerase optimized for fast and convenient high fidelity PCR
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Descrição detalhada : KOD One(TM) PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5'->3' polymerase activity and 3'->5' exonuclease activity. Upon heat-activation, the 3'->5' proof-reading ability of the enzyme generates blunt-end PCR products.
Sinónimos : Fast Hot-start PCR; High-fidelity DNA polymerase; KOD One(TM) PCR Ready Mix
Armazenamento : -20C
Embalagem : 5X1ML