LentiBrite EB3-GFP Lentiviral Biosensor

Descrição detalhada : Microtubules are dynamic cytoskeletal filaments comprising a core of tubulin subunits flanked by structurally and functionally distinct termini, termed the plus-end and minus-end. The dynamic instability of microtubules, consisting of cycles of growth and decay, occurs at the plus-ends and is mediated by a complex of microtubule plus-end tracking proteins (+TIPs). Key components of the +TIP complex are the end-binding proteins (EBs). Imaging of plus-end growth in live cells expressing EBs tagged with fluorescent proteins has contributed greatly to our understanding of microtubule dynamics. EMD Millipore's LentiBrite EB3-GFP lentiviral particles provide bright fluorescence and precise localization to enable live-cell analysis of microtubule plus-end dynamics in difficult-to-transfect cell types., Read our application note in Nature Methods!http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8620.pdf(Click Here!)Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click HereBiosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity. EMD Millipore is introducing LentiBrite Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.• Pre-packaged, fluorescently-tagged with GFP & RFP • Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods • Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells • Non-disruptive towards cellular function EMD Millipore’s LentiBrite EB3-GFP lentiviral particles provide bright fluorescence and precise localization to enable live-cell analysis of microtubule plus-end dynamics in difficult-to-transfect cell types.
Armazenamento : -80°C
Embalagem : 1X1EA