Light Diagnostics Adenovirus DFA, ~50 tests

Descrição detalhada : Light Diagnostics Adenovirus Direct Immunofluorescent Assay (DFA) Kit is intended for in vitro diagnostic use in the qualitative confirmation of adenovirus in inoculated cell cultures. Test Principle: The Light Diagnostics Adenovirus DFA Kit utilizes a direct immunofluorescent antibody technique for identifying virus in infected tissue cultures. The antibody is labeled with FITC (fluorescein isothiocyanate) which produces an apple green fluorescence when illuminated with ultraviolet light. The labeled antibody will bind to antigen present in the specimen. The FITC label on the antibody allows the antigen / antibody complex to be visualized by fluorescence microscopy. Positive specimens exhibit apple green cell fluorescence while non-infected cells are stained a dull red due to the presence of Evans Blue in the conjugate diluent. The quality of results will depend on a variety of factors such as the condition of the cells for culture confirmation, the fixative used, and the expertise of the technician performing the test. Summary and Explanation: Adenoviruses are responsible for a significant number of clinical respiratory illnesses. Upper respiratory diseases caused by adenovirus include colds, pharyngitis, and tonsillitis and occur mostly in infants and young children. Approximately 10% of childhood pneumonia is probably caused by adenovirus1. Other adenovirus related lower respiratory illnesses include bronchitis and bronchiolitis2. In children under 5, adenovirus is responsible for about 5% of cases of acute respiratory disease (ARD). ARD may be manifested by nasal congestion, coryza, cough and, at times, tonsillitis, fever, and myalgia. The appearance of conjunctivitis with ARD constitutes pharyngoconjunctival fever11. Adenovirus has commonly been associated with pertussis syndrome but recent studies suggest that the presence of adenovirus in these cases may be a reactivation of latent virus from tonsillar tissue during Bordetella pertussis infections3. Ocular illnesses resulting from adenovirus infection include epidemic keratoconjunctivitis (EKC), acute hemorrhagic conjunctivitis, and acute follicular conjunctivitis. Adenovirus is related to several gastrointestinal disorders and is probably evident in 7-17% of all childhood gastroenteritis. Types 40 and 41 have been associated with diarrhea and acute gastroenteritis4,5,6. Adenovirus has also been linked with intussusception7, acute hemorrhagic cystis8, and meningoencephalitis. Research indicates that the incidence of adenovirus infection in immunocompromised patients is probably no higher than in normal individuals, however, severity and probability of death may be greater9. Human adenoviruses belong to the family Adenoviridae, genus Mastadenovirus. They are non-enveloped, double stranded DNA viruses icosahedral in shape ranging from 70-90 nm in diameter2. Adenovirus can be cultured and isolated in a variety of cell lines and identified by several methods. Suitable cell lines include Hep-2, HeLa, KB, and HEK. Graham 293 cells may be used for propagation of types 40 and 41. Confirmation of infection is usually achieved by immunofluorescence or enzyme immunoassay but it can be done by complement fixation, hemagglutination-inhibition, and neutralization methods10., Diagnostics Kit
Armazenamento : NoTempLimit
Embalagem : 1X1EA