MN9D Mouse Dopaminergic Neuronal Cell Line

Descrição detalhada : The hybrid MN9D immortalized dopaminergic neuronal cell line was generated by fusion between rostral mesencephalic neurons from a 14-day-old C57BL/6J mouse embryo and N18TG2 neuroblastoma cells, a sympathetic nervous system cancer of A/Jax background.1 MN9D cells produce dopamine (DA) and express tyrosine hydroxylase (TH) as well as aromatic amino acid decarboxylase (AADC).2 MN9D cells readily aggregate with one another or other embryonic brain cells2 and can be transfected using calcium phosphate precipitation3 or Lipofectamine4.Undifferentiated or differentiated MN9D cells are extensively used to model dopaminergic neurons and to test mechanisms and potential therapeutics relevant to the loss of DA neurons in Parkinson’s disease. Differentiated with butyric acid or glial cell line-derived neurotrophic factor (GDNF) followed by butyric acid, MN9D cells only partially recapitulate the electrophysiological properties of midbrain DA neurons. Optimizing MN9D differentiation further using one or a combination of growth or other factors may yield an improved model system for Parkinson’s disease studies in vitro.5
Sinónimos : MN9D Mouse Dopaminergic Neuronal Cell Line; MN9D Mouse Dopaminergic Neuronal Cell Line
Armazenamento : <= - 140C
Embalagem : 1X1EA