Monoclonal Anti-beta-Actin antibody produced in mouse

Descrição detalhada : Recognizes an epitope located on the N-terminal end of the beta-isoform of actin. It specifically labels beta-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with beta-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin's solutions may be also used as fixatives. The antibody can be used as probes for beta-actin as an internal control in immunoblotting., Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three ? actins (skeletal, cardiac, smooth), one ? actin (?-non-muscle) and two ? actins (? smooth muscle and ? non-muscle). ACTB (actin ?) gene codes for ?-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.Monoclonal Anti-?-Actin (mouse IgG2a isotype) is derived from the AC-74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Sinónimos : Actin Antibody Sigma; Beta Actin Antibody; Beta Actin Antibody - Monoclonal Anti-beta-Actin antibody produced in mouse; Beta Actin Antibody Sigma; Sigma Beta Actin Antibody
Armazenamento : -20C
Embalagem : 1X100UL