Monoclonal Anti-Biotin-Agarose antibody produced in mouse

REF : A1559-.5ML
Marca : Sigma-Aldrich
Descrição :Monoclonal Anti-Biotin-Agarose antibody produced in mouse clone BN-34, purified immunoglobulin, PBS suspension
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Descrição detalhada : The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates., Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Sinónimos : Monoclonal Anti-Biotin
Armazenamento : 2-8C
Embalagem : 1X.5ML