Monoclonal Anti-Phosphotyrosine antibody produced in mouse

Descrição detalhada : Phosphorylation, attachment of a phosphate group to protein, alters protein functionality by activation or deactivation of the protein, in which protein kinase plays a crucial role. Tyrosine residue phosphorylation plays a key role in cell growth and differentiation. The monoclonal anti-phosphotyrosine antibody is useful in immunoblotting for identification of phosphotyrosine containing protein from cultured human epidermoid carcinoma cell line A-431 and human platelets preparation. This product can be used in immunofluorescent labeling of tyrosine residue at focal adhesion and cellular junctions of cultured MDCK cells. This product is also useful in studies of signal transduction and growth factor receptors. This antibody has shown specificity for binding to proteins that contain phosphorylated tyrosine residue either in free amino acid form or in conjugation with KLH or BSA but will not react with non-phosphorylated tyrosine or other phosphorylated proteins and amino acids. It will also not react with phosphorylated molecules like ATP or AMP. Monoclonal anti-phosphotyrosine reacts specifically with mouse and human., Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse., As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
Sinónimos : Monoclonal Anti-Phosphotyrosine; Phospho-Tyr; Phospho-tyrosine; p-Tyr
Armazenamento : -20C
Embalagem : 1X.2ML