MS5-hDLL1 Stromal Cell Line

Descrição detalhada : In vivo, T-cells develop in the thymus from bone-marrow derived hematopoietic stem and progenitor cells (HSPCs). In vitro, various model systems have been employed to mimic thymic function supportive of T-cell development including the OP9-DL1 system. Recently, a serum-free artificial thymic organoid (ATO) system was developed that supports highly efficient and reproducible in vitro differentiation and positive selection of human T-cells from multiple sources of HSPCs, including cord blood, bone marrow and peripheral blood CD34+ HSPCs (Seet et al. 2017). A key component of the ATO system is the MS5-hDLL1 cell line which obviates the need for primary thymic tissues., To generate the MS5-hDLL1, a murine stromal cell line (MS5) was transduced with a third-generation lentiviral vector encoding human DLL1 and eGFP (Seet et al. 2017). The highest 5% GFP-expressing cells were FACS-sorted and expanded. To form organoids, MS5-hDLL1 stromal cells are aggregated with HSPCs by centrifugation and cultured on a cell culture insert at the air-fluid interface. Detailed protocols for setting up the artificial thymic organoid (ATO) system are described in reference (Seet et al. 2017).Reference:Seet, C.S., He, C., Bethune, M.T., Li, S., Chick, B., Gschweng, E.H., Zhu, Y., Kim, K., Kohn, D.B., Baltimore, D. and Crooks, G.M. (2017) Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids. Nat Methods 14(5): 521-530.
Sinónimos : MS-5-hDLL1; MS-5-hDLL1
Armazenamento : -140C Degree Freezer
Embalagem : 1X1EA