MultiDsk-Ubiquitin-binding protein reagent

Descrição detalhada : Product Source: Yeast protein, with a GST tag and a His tag, expressed in e. coli., MultiDsk - Ubiquitin-binding protein reagent is a GST fusion protein containing an array of five ubiquitin-associated (UBA) domains from the yeast Ub-binding protein Dsk2 (aa 327-373; UniProt P48510). MultiDsk binds and protects ubiquitinated proteins from deubiquitination. When immobilized on glutathione resins, MultiDsk can be used for affinity pull-down of mono- and poly-ubiquitinated proteins from cell extracts and tissue homogenates. This construct also contains a C-terminal 6xHis sequence and an internal 6xHis sequence between the N-terminal GST and the 5XUBA sequence (PMID 23056298). MultiDsk is expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956).
Sinónimos : Dsk-Ubiquitin-binding protein reagent, Ubiquitin domain-containing protein DSK2
Armazenamento : -80°C
Embalagem : 1X1EA