Ni Sepharose(R) Excel, 25 ML

Descrição detalhada : Ni Sepharose(R) excel is an immobilized metal ion affinity chromatography (IMAC) resin precharged with nickel ions very strongly bound to a chelating ligand. This histidine-tagged (his-tagged) protein purification medium ensures minimized Ni-leakage and maximized protein recovery when samples contain stripping agents. Samples that usually cause stripping of metal ions can therefore be loaded to the medium. The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours in 10 mM EDTA. Ni Sepharose excel is designed primarily for capture and purification of his-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells by IMAC.Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures. The flow properties of the medium make it excellent for purifications in a wide range of scales and allow loading of large sample volumes, enabling purification of low concentrations of target proteins at large volumes.
Sinónimos : Ni Sepharose(R) Excel
Armazenamento : Room Temperature
Embalagem : 1X1EA