OxyIHC Oxidative Stress Detection Kit

Descrição detalhada : The OxyIHC Oxidative Stress Detection Kit (S7450) contains the chemical and immunological reagentsnecessary to detect protein oxidation in various tissues from a variety of organs and animal species. TheOxyIHC test method involves chemical derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNPat their carbonyl sites. The DNP-derivatized proteins are then detected using an antibody that specificallybinds to the DNP moiety. Subsequent incubation with biotin conjugated secondary antibody, streptavidinconjugated HRP, and development using a 3,3' diaminobenzidine (DAB) staining allowsimmunohistochemical detection of protein oxidation., Reactive oxygen species (ROS) have been implicated in pathological processes including cancer,apoptosis, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, andcardiovascular diseases (for reviews, see ref. 1-4). Living organisms are continually exposed to potentiallyharmful oxygen free radicals that are generated thru normal cellular functions as well as from environmentalfactors (1, 5-7). Yet, these reactive molecules are mitigated by the presence of antioxidants and thereforeorganisms exist with a delicate balance between oxidants and antioxidants (7-9). During times of oxidativestress there is an imbalance in favor of the oxidants. The end result of which is that free radicals are able toattack and modify sub-cellular components including nucleic acids, lipids and proteins (7, 10-12).Proteins are one of the major targets of reactive species. Oxidation of proteins modifies the side chains ofmethionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation ofproteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonineresidues in a site-specific manner (16, 20-22). The oxidative modification of proteins can modulatebiochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities oftranscription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While arelationship between protein oxidation and aging has been suggested (29-31), little is known about theimportance of oxidative modification of individual proteins in the pathophysiology of free radical mediatedprocesses. Not surprisingly, carbonyl modification of proteins has become a key biomarker for theidentification of oxidative stress.
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Embalagem : 1X1EA