PCR Master

Descrição detalhada : The PCR Master contains all the reagents required to perform a standard PCR. All that must be added is the template DNA, the primers, and the required amount of water. The PCR Master contains a fixed MgCl2 concentration of 1.5 mM. However, higher concentrations may be achieved by adding additional MgCl2. PCR products generated by PCR Master are 3'-A single-overhang products. Therefore T/A cloning can be applied.The PCR Master is stable at +2 to +8 C, allowing immediate PCR without the time-consuming thawing of reagents., Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75C and pH 9. The 5' -> 3' DNA polymerase lacks 3' -> 5' exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD.Lack of restriction endonuclease:The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
Sinónimos : PCR
Armazenamento : -20C
Embalagem : 1X1EA