PMC42-LA Human Breast Cancer Cell Line

Descrição detalhada : Human breast carcinoma cell lines are useful models to investigate the pathogenesis of neoplastic transformation and aid in the development of novel therapeutic strategies. Nonetheless, there are few well characterized cell lines derived from human mammary carcinomas and loss of breast-specific marker expression and the emergence of dedifferentiated phenotypes over extended culture often limit their use .Initiated from a pleural effusion of a metastatic breast cancer patient, the human mesenchymal breast carcinoma cell line PMC42 (also known as PMC42ET), is unique in that these cells remained pleomorphic even after several years of sustained culture . Typically grown in monolayer, PMC42 continue to give rise to numerous morphologically different cell types of both secretory and myoepithelial characteristics and this retained ability to differentiate in vitro earned their designation as stem cell like ., PMC42-LA is a stable epithelial variant of the mesenchyme-like parental PMC42 /PMC42-ET cell line. PMC42-LA congregate into pavement epithelial sheets at high density, in which the cell-cell borders contain E-cadherin and -catenin, albeit 5-10% of cells do express the mesenchymal marker vimentin. When stimulated with epidermal growth factor (EGF), PMC42-LA cells undergo an epithelial-mesenchymal transition in which vimentin expression is upregulated and E-cadherin expression is reduced .
Sinónimos : PMC42LA Cell Line
Armazenamento : liquid N2 (-196°C)
Embalagem : 1X1EA