pPolh-FLAG(TM)-1 Transfer Vector

REF : T6824-20UG
Marca : Sigma-Aldrich
Categoria :
Descrição detalhada : The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome.
Sinónimos : ddddk tag; dykddddk tag
Armazenamento : -20C
Embalagem : 1X20UG