Proteinase K, recombinant, PCR Grade

Descrição detalhada : Proteinase K is a subtilisin-related serine protease. The recombinant enzyme is identical to the native protease originally isolated from the mold Tritirachium album. The specifications of the recombinant enzyme are the same as those of the native protease. The amino acid sequence (molecular weight) and the molecule structure are identical. However, the recombinant preparation is much purer than the native enzyme, as it is DNA-free that has no pronounced cleavage specificity. The enzyme is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. The enzyme is also available as a solution.. Inhibitors: The enzyme is inactivated by Pefabloc(R) SC. However, it is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.. Activators: Proteinase K activity is stimulated by the presence of denaturing agents (SDS and urea).. pH Optimum: Proteinase K is stable over a wide pH range (4 - 12.5). The enzyme maintains full activity for several hours when incubated at a pH between 6.5 and 9.5.. Volume Activity: Approximately 2.5 U/mg lyophilizate (chromozyme assay)Approximately 30 U/mg lyophilizate (hemoglobin assay)Note: SDS can increase the activity of Proteinase K as much as sevenfold.
Sinónimos : pro k; proteinase K
Armazenamento : 2-8C
Embalagem : 1X1EA